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Nano or No-No? Hydroxyapatite Particle Size Matters…

In this article, we’re going to take a deep dive into hydroxyapatite. We’ll cover what it is, how beneficial it is to help stop tooth decay, and the potential risks of nano-hydroxyapatite.

Fair warning, this article is going to be a deep dive that provides a lot of details about hydroxyapatite.

So if you’re new here and you’d like a more high-level introduction to remineralization or hydroxyapatite’s role in helping to stop and/or protect against tooth decay, these articles may be an easier read:

  • How Teeth Decay
  • Putting Together the Pieces to Reverse Tooth Decay and Remineralize Cavities

In 2016, we launched our Shine Remineralizing Tooth Whitening Powder, which contains naturally sourced hydroxyapatite (it’s actually the #1 ingredient in Shine 🙂 ). Since then, many other companies have developed oral hygiene products using various forms of hydroxyapatite.

It makes sense that hydroxyapatite’s popularity is on the rise. After all, the research clearly shows that hydroxyapatite protects against tooth decay just as well as (and sometimes even better than) fluoride.

However, like so many things in life, not all hydroxyapatites are created equal. The quality, quantity, and particle size of a product’s hydroxyapatite can all make a big difference.

Speaking of particle size, the blogosphere has recently been abuzz with debates about the risks vs. benefits of nano-sized hydroxyapatite. So, we decided to provide more information so you can make up your own mind on this developing subject.

What is hydroxyapatite?

To start, let’s make sure we’re all on the same page.

Hydroxyapatite (high-drox-ee-appetite), is the chemical name for a molecule made up of calcium, phosphorus, oxygen, and hydrogen. For you scientists (or armchair scientific types, like me), the chemical composition is Ca5(PO4)3OH. However, since it shows up as a pair in nature, it’s commonly notated as Ca10(PO4)6(OH)2. The word ‘hydroxyapatite’ is commonly abbreviated as HA.

The HA molecule is very important for oral care.

It’s the primary building block that our teeth are made of.

But for decades, instead of focusing on hydroxyapatite, we’ve been taught that we need to use fluoride to prevent tooth decay. Leaning on our high school chemistry class, we might recall that fluoride is a halide, which means it’s a super ‘attractive’ element.

When we introduce fluoride into the mouth, it binds with the calcium and phosphorus components of our teeth, which creates fluorapatite (note, not hydroxyapatite). However, our teeth do not naturally contain fluoride, and tooth decay is not the result of a fluoride deficiency. 

So, for the purposes of this article, we’ll set aside fluoride. Instead, we’ll anchor on the fact that our teeth are made of hydroxyapatite, which is why HA is such a supportive compound for tooth health.

Quick primer on particle size…

Particle sizes can be expressed using different units of measurement. For the size we’ll be dealing with here, the metric system is generally used. We’ll be talking in micrometers (also called microns) and nanometers. And for perspective, let’s zoom out and start with millimeters.

Millimeters are the tiniest marks on a standard US/metric ruler. 10 millimeters = 1 centimeter, which is about ⅓ of an inch. 

(As a side note, if you’ve had a periodontal depth test, you probably know that the numbers the dentist calls out to the assistant are in millimeters. Any gum pocket that’s deeper than 4 millimeters is problematic. Once gum pockets get deeper than that, it’s difficult to effectively maintain healthy bacterial populations. Thankfully, there’s a product solution that can help if you have deeper gum pockets. Now let’s get back to measurements…)

There are 1000 micrometers in a millimeter. 

Stepping down another rung into the hard-to-imagine range, there are 1000 nanometers in 1 micrometer.

So, that means that there are one million nanometers in one millimeter. 

Here’s the takeaway that will help simplify this: ‘nano’ is one thousand times smaller than ‘micro’. 

So for example, something that’s 5 micrometers in diameter could also be measured as 5000 nanometers. In other words, nanoparticles are WAY smaller than microparticles.

To look at it another way, if a golf ball were one nanometer in diameter, a micrometer would be nearly half a football field in diameter, and one millimeter would be just over 23 miles. So, nanometers are 1000 times smaller than micrometers, which are 1000 times smaller than millimeters.

Particle size matters…

Hydroxyapatite can come in many different particle sizes. For medical, dental, and oral supplement applications, particle sizes range from about 800 micrometers all the way down to 10 nanometers (80,000 times smaller).

To assist with optimal uptake in the mouth, we want the particle size to be pretty tiny. That way, it can go into suspension with our saliva and be available to deposit more minerals into and onto our teeth. (For more info on how this works with Shine, please take a look at this video.)

The naturally sourced hydroxyapatite we use in Shine is pharmaceutical-grade bone powder from grass-fed New Zealand cattle. 

(Incidentally, the same hydroxyapatite that we use is also used in the supplement field as a bioavailable source of calcium. In fact, there’s substantial evidence that suggests that the body uptakes HA better than other forms of calcium.) 

We use the finest particle size our team can mechanically produce, which is right around 70 micrometers. To differentiate it from nano-HA, we refer to our hydroxyapatite as MCHA (microcrystalline hydroxyapatite) with the ‘micro’ distinguishing the particle size of the HA.

But if a tiny size is better, why not use the smallest possible particle size?

As we go from micrometers to nanometers, we have to think about what might happen in the body if we use an ingredient with such a tiny particle size.

Nano or no-no?

If you’ve been reading our material for a while, you already know that OraWellness is hardwired to a holistic paradigm. This affects many of our decisions, especially when it comes to product development.

When we were doing the R&D for Shine, we saw that the research literature supported using nano-HA for helping to restore demineralized areas in teeth. But we also saw some bad PR about nano-sized particles indiscriminately traveling throughout the body (due to their extremely tiny particle size).

In our opinion, nano-HA had two things going against it:

  1. The particle size was so tiny that the molecule was able to easily migrate throughout the body (for example, across the blood-brain barrier and even into the cellular structure), and literature was emerging that suggested that this wasn’t safe.
  2. Nanoparticles are synthetically produced (not naturally derived).

So, with our holistic paradigm guiding us, we chose to apply the ‘precautionary principle’ and avoid nano-HA until we knew for certain that it was safe to use long-term.

(For more info on our thought process in these situations, check out our simple, two-step exercise that you can apply to any hygiene product to determine if it’s holistic or not.)

What are the risks with nano-HA?

In the EU, there’s a General Product Safety Directive that says, “Businesses must only place products which are safe on the market…” But, nano-HA hasn’t been proven to be safe.

In fact, earlier this year, the European Union announced that it plans to ban several nano-sized materials (including nano-HA) for use in cosmetic and oral hygiene applications. Here’s a link to the full report on nano-HA from the EU’s Scientific Committee on Consumer Safety (SCCS).

The SCCS is especially concerned about the potential for nano-HA to damage cell DNA. The term they reference is ‘genotoxic potential’, which means the possibility for something (in this case, nano-HA) to damage the genetic information within a cell, causing mutations that may lead to cancer. 

In their report, the SCCS states, “Having considered the data provided, and other relevant information available in scientific literature, the SCCS cannot conclude on the safety of the hydroxyapatite composed of rod-shaped nanoparticles for use in oral-care cosmetic products at the maximum concentrations and specifications given in this Opinion. This is because the available data/information is not sufficient to exclude concerns over the genotoxic potential of HAP-nano.”

And their hesitancy seems warranted for other reasons, too. Other studies point to issues like the risk that nano-HA may cause cellular breakdown in kidney cells and that it may even have a negative impact on human blood cells.

All of this provides plenty of reasons for us to continue to apply the precautionary principle and steer clear of nano-HA. 

But what does the SCCS think about NON-nano-HA?

In section 3.3.4 Assessment of the systemic toxicity of the non nanoform of HAP, the report says, “Calcium phosphate is a common mineral on earth and the most common calcium phosphate mineral is hydroxyapatite. Calcium phosphates have been generally recognized as safe (GRAS) in food by the FDA in 1975. Calcium phosphate is highly biocompatible in contact with hard tissue because the body is well accustomed to this mineral.”

Thank goodness we can still benefit from using hydroxyapatite without the risks of using nanoparticles.

Is microcrystalline HA as effective as nano-HA?

There are two ways science seeks to prove or disprove any hypothesis: via clinical trials or via empirical evidence (any patterns or behaviors found during personal observation or experimentation).

Let’s start with what the research currently says about the efficacy of micro-HA.

To our knowledge, there are no research studies comparing different sizes of HA against one another. Here’s a study that mentions both micro- and nano-HA, but we haven’t yet found data that compares the efficacy of various particle sizes of HA.

However, here’s a well-done study that compares micro-HA to the gold standard of conventional dentistry: 1400 ppm (particles per million) fluoride. The researchers chose to study individuals with braces (which is a group that’s at high risk for decay). Here’s the takeaway:

“In highly caries-active patients, the impact of the regular use of a microcrystalline HAP dentifrice [toothpaste] on caries [cavities] progression is not significantly different from the use of a 1400 ppm fluoride toothpaste.”

At first blush, that might not sound very noteworthy. But, the data in that study actually shows that the participants who used a micro-HA toothpaste had less decay than those who used a fluoride-based toothpaste.

And, this one-year, double-blinded, randomized controlled trial tested whether micro-HA protects children against early cavities as effectively as fluoride-based toothpaste. It found, “This RCT [randomized controlled trial] showed for the first time, that in children, the impact of the daily use of a toothpaste with microcrystalline hydroxyapatite on enamel caries progression in the primary dentition is not inferior to a fluoride control toothpaste.”

And what about empirical evidence?

Well, we have plenty of that from the thousands of testimonials we’ve received from you, our community, through the years since we launched Shine, our remineralizing tooth whitening powder with MCHA.

We love all of the testimonials we regularly receive. Some of them are jaw-droppers, like this one:


“I just found out one of the reasons I struggled with cavities for years: I have an autoimmune disease which causes dry mouth, and was depleted by stress and further health issues. I ended up with 11 cavities a few years ago despite great oral hygiene. I finally ordered many of your products at that time, including several containers of Shine.

Within 3 months, 10 of the cavities were gone and only the oldest deepest one needed a filling. The hygienist thought I must have had those cavities filled elsewhere as they were gone.

I have continued to use your products, and in the 3 years since then, have no new cavities, continued great gum health, and very little plaque at my dental visits. I love the smooth feel of the oil blend on my teeth, and it feels good to know that I can prevent cavities despite this autoimmune condition. Thank you!”

– Tara B. from Canada

This is empirical evidence, plain and simple. And we’re honored that so many of you have used our product solutions to create greater oral health for yourself. That’s dental self-empowerment.

Wrapping up…

Thanks for joining us for this fascinating deep dive on hydroxyapatite and particle sizes. For more information on hydroxyapatite as well as other in-the-mouth and whole-body strategies for helping teeth remineralize, feel free to download our free eBook, How to Stop Tooth Decay and Remineralize Your Teeth.

What about you, what are your thoughts on nano- vs. microparticles? Are the benefits of using nano-HA greater than the risks? Ultimately, it becomes a personal value judgment. We hope this information helps you to make educated decisions on what ingredients to use in your oral hygiene strategy. Please share your thoughts and experiences in the comments below so we can all learn from each other.

Helpful, related resources:

  • What makes our MCHA stand out from the crowd? [informational resource]
  • How Teeth Decay [article]
  • Putting Together the Pieces to Reverse Tooth Decay and Remineralize Cavities [article]
  • Shine—Remineralizing Tooth Whitening Powder with Hydroxyapatite [product solution]
  • Is Fluoride Safe to Use? [article]
  • One Test You Definitely Want to Get from Your Dentist [article]
  • Gum Disease Solution: The HealThy Mouth System [product solution]
  • How Shine Works [video]
  • How to Tell if Your Oral Hygiene Products are Holistic or Not [article]
  • How to Stop Tooth Decay and Remineralize Your Teeth [FREE eBook]

Other resources:

  • https://pubmed.ncbi.nlm.nih.gov/?term=hydroxyapatite+remineralization
  • European Commission Scientific Committee on Consumer Safety Opinion on Hydroxyapatite (Nano)
  • EU General Product Safety Directive
  • Study: Effects of physical properties of nano-sized hydroxyapatite crystals on cellular toxicity in renal epithelial cells
  • Study: Evaluation of cytotoxic, oxidative stress and genotoxic responses of hydroxyapatite nanoparticles on human blood cells
  • Study: The use of hydroxyapatite toothpaste to prevent dental caries
  • Study: Impact of a non-fluoridated microcrystalline hydroxyapatite dentifrice on enamel caries progression in highly caries-susceptible orthodontic patients: A randomized, controlled 6-month trial
  • Study: Impact of a toothpaste with microcrystalline hydroxyapatite on the occurrence of early childhood caries: a 1-year randomized clinical trial

Categories

Mouth/Body Connection, Oral Hygiene, Remineralization, Tooth Decay, Toxin Free Oral Care

Tags

mouth/body connection, oral hygiene, tooth decay

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Comments

  1. Amanda says

    August 18, 2022

    So what size is too small exactly? Anything less than one micron?

    Reply
    • Chad at OraWellnesss says

      August 23, 2022

      Aloha Amanda,

      Great question!

      Our assessment of the available research is that particles ≤ about 200 nanometers should not be consumed.

      The European Commission defines a nanoparticle as a particle that ranges between 1-100 nanometers in size (source).

      Based on that definition, anything above 100 nm in size could technically be termed “non-nano”. It’s great that the European Commission gives a guideline, but there’s some gray area here.

      For example, one company we corresponded with stated that the lowest particle size in their product is 200 nm, which is technically “non-nano”. However, this company couldn’t guarantee that their product is “non-nano” because whenever you powder something, you’re going to get a wide range of particle sizes, so there’s always a chance that very fine particles (even nano-sized) are present when you’re powdering a substance down to the 100-200 nanometer range.

      From our view, the best / safest path forward is to minimize the concentration of nanoparticles by choosing larger size cuts of hydroxyapatite. This will give more “buffer room” with the particle size distribution.

      In case you’d like to take a deeper dive, here’s an article on nanoparticles: Nanoparticles – known and unknown health risks

      I hope that helps! 🙂

      Reply
  2. Sondra Hamm says

    August 11, 2022

    Thank you so much!!

    Reply
  3. Crystal S says

    July 31, 2022

    Hi! I have a couple questions. Will you ever consider coming out with a toothpaste containing HA? My children and even for myself prefer the texture of toothpaste over tooth powder. Also, my shine powder got some moisture and is clumpy. I actually find it easier to use than the dry powder that hardly wanted to stay on my brush. Is it still effective? The reason I’m asking is cause you are always warning against getting moisture in it. Thanks!

    Reply
    • Chad at OraWellnesss says

      August 2, 2022

      Aloha Crystal!

      We’re actually currently developing a custom toothpaste with hydroxyapatite. 🙂 Since you’re already signed up for our emailing list, you’ll be among the first to know when we’re ready to offer this new product to our community.

      In the meantime, you might find it helpful to know that you can mix our Shine Tooth Powder and/or our HealThy Mouth Blend with some organic coconut oil to form a paste that you can brush with (coconut oil has plenty of antimicrobial and anti-fungal properties to support oral health). Alternatively, you / your children could try mixing some Shine Powder with your favorite toothpaste.

      Thankfully, Shine is still effective, even when moisture winds up in there and it becomes gooey or clumpy. Feel free to use a butter knife, fork, or another kitchen tool (maybe a potato masher, a mortar and pestle, or a blender?) to break the Shine into smaller clumps and then mix it with some organic coconut oil to turn it into a paste that you can brush with. Then you just need to use a small dab of the paste each time you brush (that way, you don’t go through it too quickly).

      I hope that helps. Aloha! 🙂

      Reply
  4. Adam says

    July 31, 2022

    Would the RiseWell toothpaste be a good product to accompany my oral wellness protocol, I noticed it contains Hydroxyapatite. Any assistance or reply is greatly appreciated.

    Reply
    • Chad at OraWellnesss says

      August 2, 2022

      Aloha Adam,

      Thanks for stopping by. 🙂

      We can’t speak much on the efficacy of other products. That said, one of our customers recently let us know that they emailed RiseWell to ask about the particle size of their hydroxyapatite and RiseWell replied to say that it is 5 micrometers.

      As you may already know, the particle size of our MCHA is around 70 micrometers (i.e. 70,000 nanometers). Based on our research, using a hydroxyapatite with a smaller particle size of around 5 micrometers (i.e. 5,000 nanometers) would also be okay / safe.

      I hope that helps. Aloha! 🙂

      Reply
  5. Ellie says

    July 31, 2022

    Very informative and theses concerns about nano HA appear very real.
    I heard that HA attracts fluoride to itself & the product has been used to remove Fluoride from soil.
    What do you know about the presence of Fluoride being incorporated into HA or fluoride being attracted to HA? ( possibly from tap water etc).

    Reply
    • Will And Susan says

      August 6, 2022

      Aloha Dr Ellie!

      Great to hear from you! It’s always an honor to touch base with you.

      Hmm, we’re not aware of any data of HA attracting fluoride to itself. As HA is a stable structure, my guess is more than anything that fluoride is just so darn chemically ‘attractive’ that just about anything will engage with it. What you say here about using HA to ‘draw’ fluoride from soil and water sounds possible. Maybe rather than using HA, if it were just the apatite molecule (without the hydroxy OH on it), that would provide the chemical space for fluoride to attach and create ‘fluorapatite’ (aka what fluoride treatments do in/on our teeth when applied there). Again, I’m way outside of my skills here, so it’s just a guess.

      If anyone has more info on this, post it so we can all learn together!

      Thanks again!!

      Reply
  6. Cheri says

    July 31, 2022

    Thanks for the great article.
    I wonder how people can detox from the already absorbed nano HA…any ideas. One of my family members has used two tubes on nano-HA toothpaste before I told him to stop. I hope his body will gradually get rid of it since he is no longer using it.
    Another question of mine:
    I am very interested in your Shine product but don’t use it because I am a vegetarian. On the other hand, I’ve read that micro-HA is also being made from eggshells. It would be AWESOME if you could offer such a product in the future. Then lots of vegetarians like me would buy it from you. I love your other products and use them regularly.

    Reply
    • Will And Susan says

      August 6, 2022

      Hi Cheri,

      Thank you for your kind words and support.

      Hmm, I haven’t heard anything about strategies to dexox nano particles. However, one of our team is a chemist and has substantial experience from past work with nano particles. So I’ll ask her and if I gather any gems, I’ll circle back here and post them.

      Re: Shine – Yes, we understand that there are many vegetarians/vegans who need support like Shine provides. All I can say right now is, we’re very aware of this and actively working on a solution (shh :).

      We encourage you to keep an eye on our emails in the coming months as we’ll have some very exciting news for you and others.

      Thanks again!

      Reply
  7. Ron says

    July 24, 2022

    I’ve had some discussions with people over nano-HA who think it’s perfectly safe because the digestive tract dissolves nano-HA. But we need to consider that what we put in our mouth or up in your gums can end up in our bloodstream so that should always be in the back of your mind. I am not comfortable at all with the idea of nano-HA being in my bloodstream. People point to the widespread use of nano-HA toothpaste in Japan as evidence of its safety, but since Japan’s health outcomes are being compromised by things like the adoption of western diet (such as heart disease on the rise) I can’t really rely on that since there’s no way to actually know what nano-HA might or might not be contributing to. Also, I’m not going to name any names but one of the most popular nano-HA toothpaste manufacturers in the USA is in my opinion shady on this topic when I tried to engage them about my concerns in a friendly way. They just replied to me “google it” rather than support their own stance and commitment to safety. We cannot blindly trust alternative toothpaste manufacturers to have our best interests in mind. They are out to make $$$. I’m glad you guys played it safe with micro-HA and are transparent and engage with your customers concerns!

    Reply
    • Will And Susan says

      July 24, 2022

      Thanks so much for offering your perspective here, Ron.

      Really great points all around. We agree that the tests in Japan for years have supported the use of nano-HA. And yet, as so often is the case, how the researchers (or those funding the study) frame and structure a study is going to have a huge impact on what gems, if any, come from the study. The recent studies on the whole body impacts of nano-HA certainly are enough for us to have a wary view on this tech.

      And thanks for the feedback about our transparency. We really do aspire to listen to the needs and concerns of our community and engage our efforts from there. After all, we wouldn’t be here if it weren’t for community like you appreciating our work! 🙂

      Thanks again Ron.

      Reply

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Shine—Remineralizing Tooth Whitening Powder w/ Hydroxyapatite

https://store.orawellness.com/products/shine-remineralizing-tooth-whitening-powder

“Excellent product. My mouth feels refreshed and clean using this powder. I feel like I’m taking good care of my oral health with this. There are no harmful ingredients and it helps to remineralize my teeth.” – Mary C. from Canada

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Since I grew up in the generation that encouraged honey in a baby bottle, “penny candy” and a daily candy bar in my lunch box….I have had cavities my entire life! I just became used to yearly shots of novacaine!

Since making a healthy lifestyle change 6 years ago which also included dental awareness & health, I began using Ora Wellness Healthy Mouth Blend and most recently “Shine”!

I can’t remember the last cavity & I even receive compliments from my Dentist & hygienist on how healthy my teeth & gums are!

On my last visit my Dentist said, “Whatever you are doing…just keep it up!”

My husband & friends compliment my smile & white teeth consistently!

In the words of Soupy Sales, “Be true to your teeth & they won’t be false to you!”
Thank you Ora Wellness….couldnt have done it without you!

Lisa H from Bonsall, CA

I first encountered OraWellness through the Heal Thy Mouth blend. I loved the way my mouth felt after using it and enjoyed not making my own toothpaste! My gums are healthier than they’ve ever been.

I was going to start making my own toothpaste again to heal my teeth and remineralize them. I was happy to find the Shine tooth powder instead! I also love the way my mouth feels after using it and sensitivity has decreased with use!

I look forward to continuing results!

Sarah M from Ewa Beach, HI

I’ve been making my own tooth powder for over a year now, with good results. I’ve also tried other natural tooth powders that were just okay. I decided to try Shine because I used OraWellness Healthy Mouth Oil alongside my toothpowder and on my floss with amazing results, that even my dentist noticed right away.

Shine is just amazing. It’s a step beyond my own tooth powder that I make. I thought it couldn’t get any better than my own homemade, but Shine is something very stellar!

I noticed a difference after just a couple of times of using it. My teeth are brighter and whiter and I love the taste as well. The bright smile I have now makes me look a lot younger than I really am! Who doesn’t want that??!

I still use the Healthy Mouth oil on my floss and I use it in water as a rinse, but Shine does everything and more that a tooth powder should do. So happy with the results!

Jeanie K from Highlands Ranch, CO

I just want to say thank you for once again taking time to make a beneficial and safe product!

The Shine powder is amazing of course. It’s not overpowering in flavor and not a gritty feel at all. I really appreciate the fact that it can be applied many ways as suggested and also turned into a paste for a thicker application.

It has furthered my journey toward natural healthy teeth and can’t wait to see more! Thank you

Trisha H from Woodland, WA

I’ve been making my own tooth powder for years, but I was surprised that my teeth were still turning yellow! There seemed to be quite a thick stained layer especially on my front two teeth. I thought a dental hygienist would need to scrape it off for me.

But after using Shine for a few days my teeth felt really smooth and noticeably whiter. Now after a few weeks I am so excited about the results! I do not need anyone to polish my teeth.

This stuff is awesome!!! It is remineralizing my teeth and making them SHINE!

Lisa G from Kentwood, MI

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